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Image Search Results
Journal: Fems Microbiology Letters
Article Title: Evidence for recombination between a sialidase ( nanH ) of Actinomyces naeslundii and Actinomyces oris , previously named ‘ Actinomyces naeslundii genospecies 1 and 2’
doi: 10.1111/j.1574-6968.2008.01336.x
Figure Lengend Snippet: Neighbour-joining tree showing relationships between type and reference strains of Actinomyces naeslundii, Actinomyces oris, Actinomyces johnsonii and Actinomyces viscosus and oral and clinical isolates determined by partial nanH gene sequence analysis. Actinomyces naeslundii strains 25, 51 and CCUG 34725 and A. oris strains 60 and 61 (each shown with the nanH sequence accession number) did not cluster with the majority of strains of the same species and were identified as strains with significant evidence of interspecies recombination. Scale bar=0.01 substitutions per site.
Article Snippet: The strains included in this study were 30
Techniques: Sequencing
Journal: Fems Microbiology Letters
Article Title: Evidence for recombination between a sialidase ( nanH ) of Actinomyces naeslundii and Actinomyces oris , previously named ‘ Actinomyces naeslundii genospecies 1 and 2’
doi: 10.1111/j.1574-6968.2008.01336.x
Figure Lengend Snippet: Genetic variation of partial nanH sequences of type and reference strains and clinical isolates of Actinomyces oris and Actinomyces naeslundii
Article Snippet: The strains included in this study were 30
Techniques:
Journal: Fems Microbiology Letters
Article Title: Evidence for recombination between a sialidase ( nanH ) of Actinomyces naeslundii and Actinomyces oris , previously named ‘ Actinomyces naeslundii genospecies 1 and 2’
doi: 10.1111/j.1574-6968.2008.01336.x
Figure Lengend Snippet: Recombination events found in nanH of Actinomyces oris strains 60 (EU805671) and 61 (EU805672) and Actinomyces naeslundii strains 51 (EU805620), 25 (EU805612) and CUG 34725 (EU805609). Solid line indicates portion of sequence derived from nanH of A. naeslundii and broken line indicates portion of sequence derived from nanH of A. oris . Insertion in strain 25 between 365 and 1038, in strain 51 between 629 and 1038, in strain 60 between 1 and 477, in strain 61 between 432 and 1038 and between 1 and 655 in CUG 34725. Breakpoints determined using RDP suite of programs with significant evidence ( P <0.001) for recombination obtained with ≥5 recombination tests in all cases.
Article Snippet: The strains included in this study were 30
Techniques: Sequencing, Derivative Assay
Journal: Fems Microbiology Letters
Article Title: Evidence for recombination between a sialidase ( nanH ) of Actinomyces naeslundii and Actinomyces oris , previously named ‘ Actinomyces naeslundii genospecies 1 and 2’
doi: 10.1111/j.1574-6968.2008.01336.x
Figure Lengend Snippet: Reverse transcriptase (RT)-PCR assays showing expression of nanH and the housekeeping gene atpA in the type strains of Actinomyces oris (CCUG 34288; EU805602), and Actinomyces johnsonii (CCUG 34287; EU805600), Actinomyces naeslundii (CCUG 2238; EU805601) and Actinomyces viscosus (NCTC 10951; EU805603). Negative controls, omitting the RT in the reaction mix, yielded no amplicons from any strain.
Article Snippet: The strains included in this study were 30
Techniques: Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, Expressing